WEST TUNE QPCR Accurate 96 Real-time Quantitative PCR---PCR
West Tune QPCR Accurate 96 is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches international advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
Specifications:
Temperature control system |
Sample capacity |
0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
Reaction volume |
10-50 μl |
Thermal cycle technology |
Peltier |
Max. Heating/Cooling rate |
6.0° C/s |
Heating temperature range |
4 - 100 °C |
Temperature accuracy |
± 0.2°C |
Temperature uniformity |
±0.2ºC @60ºC , ±0.2ºC @95ºC |
Temperature gradient setting range |
30-100°C |
Temperature gradient difference setting range |
1 - 36 °C |
Detection system |
Excitation light source |
4/6 monochrome high efficiency LEDs |
Detection device |
PMT |
Detection mode |
Time-resolved signal separating technology |
Excitation/detection wavelength range |
455-650nm/510-715nm |
Fluorescent channels |
4/6 channels |
Supported dye |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
Sensitivity |
Single copy gene |
Resolution |
1.33 folds copy number difference can be distinguished in single-plex qPCR |
Dynamic range |
10 orders of magnitude copies |
Analysis mode |
Absolute quantification, Melting curve analysis, Endpoint fluorescence analysis |
Data output |
Original file, Excel data file, results include run settings, graph and tabular data results |